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3 Basic Principles of Recombinant DNA Technology

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: August 18, 2016 - Views: 3

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: August 19, 2016 - Views: 1

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: August 27, 2016 - Views: 2

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HOW TO PREPARE VECTORS TO CARRY YOUR FAVORITE...

Biology 417 Week 1, Lecture #2 With input from: Yung Huang, Luis Sanchez, Lee Lin, Leticia Argueta, Kay Nguyen PGM 2000 Revised 2012 - SBS

http://www.calstatela.edu/faculty/ssharp/slides/%21PGM2012%21/12BiotechRE2012.ppt

Date added: August 30, 2016 - Views: 1

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 18, 2016 - Views: 1

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PowerPoint Presentation

Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: August 27, 2016 - Views: 1

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Slide 1

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/ProcedureOfGene%20cloning19.21-4-2016/2%20Cloning%20vectors.pptx

Date added: September 7, 2016 - Views: 1

ppt
PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 28, 2016 - Views: 2

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: August 20, 2016 - Views: 2

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Inquiry into Life Twelfth Edition - Regis...

vectors: Plasmids; Phages . ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. ... Inquiry into Life Twelfth Edition

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 21, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

DNA cloning & cloning vectors ... gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%202%202009.ppt

Date added: August 18, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 4500 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Lecture%203.ppt

Date added: August 27, 2016 - Views: 2

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: August 30, 2016 - Views: 1

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: August 20, 2016 - Views: 1

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: August 30, 2016 - Views: 1

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Title goes in here - cmb-file.persiangig.com

Phage vectors (λ) Cosmids. ... Secretion Vectors. Formation of Inclusion Bodies. May 26, 2013. ... (shuttle vectors) Yeast Integrative Plasmids (YIp)

http://cmb-file.persiangig.com/other/Microbial%20Biotechnology.ppsx

Date added: September 24, 2016 - Views: 1

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Competent Cells formation and transformation of...

competent cells formation and transformation of competent cells ... Cosmids: an extra chromosomal circular DNA molecule that combines features of plasmids and ...

http://fac.ksu.edu.sa/sites/default/files/2-competent_cells_formation_and_transformation_of_competent_cells_with_dna_0.pptx

Date added: August 30, 2016 - Views: 1

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Vectors - College of Computer, Mathematical, and...

Recombinant DNA Technology in Today’s Medicine Shelley M. Martineau Jessica A. Matthews Catherine C. Miller Carol D. Riley Institute of TIP Productions, Inc.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: August 22, 2016 - Views: 1

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Lambda Vectors and their replication - Biology

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: August 30, 2016 - Views: 1

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DNA cloning intro.ppt - Biology

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: August 23, 2016 - Views: 2

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: August 30, 2016 - Views: 1

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: August 30, 2016 - Views: 1

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Chapter 12

... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: August 30, 2016 - Views: 1

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Setting up a transformation--how will the...

Setting up a transformation--how will the competent cells be treated? No plasmid (negative control, nothing should grow on this plate)

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: August 30, 2016 - Views: 1

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Genetic Engineering - University of North Carolina...

Genetic Engineering ... Vector Stable Packaging Cosmids Other Vectors Expression Vectors Secretion ... Splice into Cloning Vehicle Plasmids Viruses Use ...

p://people.uncw.edu/sizemorer

Date added: August 18, 2016 - Views: 1

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A DNS tulajdonságai hogyan használhatók a...

A DNS tulajdonságai hogyan használhatók a manipulációban? Könnyen izolálható, elektroforézis, Összeilleszthető Megolvasztható, kelálható festékekkel ...

http://biokemia.elte.hu/attachments/download/course/33/23

Date added: August 21, 2016 - Views: 1

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: August 30, 2016 - Views: 1

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Molecular Cloning I: Restriction Enzymes, PCR, ...

Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: August 23, 2016 - Views: 1

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Part II: Recombinant DNA Technology.ppt - Rajesh...

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://www.rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: August 30, 2016 - Views: 1

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Recombinant DNA Technology for the non-science...

Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: August 30, 2016 - Views: 1

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Enzyme Mechanisms - Illinois Institute of...

... Shuttle vectors These are plasmids that can operate in two ... Enzyme Mechanisms Author: ... cloning Use of bacteriophage lambda Cosmids ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: August 18, 2016 - Views: 1

ppt
PowerPoint Presentation

... specialized plasmids Vectors containing ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and ... Document presentation format ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: August 22, 2016 - Views: 1

ppt
Slide 1

Lecture 14 – Gene Cloning Based on Chapter 08 - Genomics: The Mapping and Sequencing of Genomes Copyright © 2010 Pearson Education Inc.

http://www.auburn.edu/academic/classes/biol/3020/lecture_ppts/L14_DNA%20Cloning_14F.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Vectors Slide 11 Plasmid Vectors Plasmids Slide 14 ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_1.ppt

Date added: September 2, 2016 - Views: 1

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Inquiry into Life Twelfth Edition -...

Lecture PowerPoint to accompany Molecular Biology Fifth Edition Robert F. Weaver Chapter 4 Molecular Cloning Methods Copyright © The McGraw-Hill Companies, Inc ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: August 30, 2016 - Views: 1

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Inquiry into Life Twelfth Edition - McGraw Hill...

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: August 30, 2016 - Views: 1

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Enzyme Mechanisms - Illinois Institute of...

... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...

http://csrri.iit.edu/~howard/biochem/lecppt/recombinantF10_401.ppt

Date added: August 18, 2016 - Views: 1

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PowerPoint Presentation

Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: August 22, 2016 - Views: 1

ppt
Slide 1

Chapter 8: Recombinant DNA Technology and Molecular Cloning

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 20, 2016 - Views: 1

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DNA Libraries - Western Washington University

... plasmids BACS YACS Transduction ... phage cosmids Cloning Step 3 ...vectors ... and provide a means of replication for itself and the foreign DNA it carries. And ...

http://www.biol.wwu.edu/young/321/LG8.ppt

Date added: September 2, 2016 - Views: 1

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Exploring Genes - faculty.msmc.edu

Exploring Genes Recombinant Technololgy ...

http://faculty.msmc.edu/turkenko/html/exploring_genes.ppt

Date added: September 8, 2016 - Views: 1

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PowerPoint Presentation - DNA Libraries

And, Vectors … …contain ... Transformation (via bacterial mechanisms); plasmids BACS YACS Transduction ... PowerPoint Presentation - DNA Libraries Author:

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: August 23, 2016 - Views: 1

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Slajd 1 - Strona Główna - Politechnika Gdańska

Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col ... Cosmids M13 Phage Vectors Copyright © The McGraw-Hill Companies ...

http://www.pg.gda.pl/chem/Katedry/Mikrobiologia/W2-IG2.ppt

Date added: August 30, 2016 - Views: 1

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Genomes 3/e - California State University, Los...

Add/Drop business Announcements Boldface terms and flashcards Quizzes start Monday. You need 2 small bluebooks. Teach for America California Institute for ...

http://www.calstatela.edu/faculty/ssharp/slides/20MG10/SBSchapter02.ppt

Date added: September 8, 2016 - Views: 1

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Part II: Recombinant DNA Technology.ppt

Recombinant DNA Technology Sherry Fuller-Espie, Ph.D., DIC Associate Professor Cabrini College © Sherry Fuller-Espie, 2003 ...

http://www.123seminarsonly.com/Seminar-Reports/029/47361166-recombinant-DNA-technology-Fuller.ppt

Date added: October 1, 2016 - Views: 1

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Table of Contents - Midwestern State University

... Phage Vector Vectors Plasmids 5-10Kbp inserts Genomic DNA or cDNAs Phage 5-20Kbp inserts Genomic DNA or cDNAs Cosmids 20 ... are used as vectors Plasmids ...

http://faculty.mwsu.edu/biology/jon.scales/Courses/POB/Lecture_Notes/recDNA05.ppt

Date added: August 30, 2016 - Views: 1

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Chapter 14

Chapter 14 Recombinant DNA ... Recombinant DNA there are four types of cloning vectors plasmids ... viruses cosmids artificial chromosomes Recombinant DNA Cloning ...

http://www2.sunysuffolk.edu/seshadp/by272/Chapter%2014.ppt

Date added: August 19, 2016 - Views: 1

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Foundations in Microbiology - South Georgia...

Foundations in Microbiology Sixth ... (plasmid, virus, cosmids) ... Characteristics of Cloning Vectors Must be capable of carrying a significant piece of donor ...

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: September 18, 2016 - Views: 1

ppt
No Slide Title

Art and Photos in PowerPoint® Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 13 Recombinant DNA Technology and Gene Cloning

http://ww2.biol.sc.edu/~awaldman/Klug9chapt13.ppt

Date added: September 8, 2016 - Views: 1