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Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 28, 2016 - Views: 1

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PowerPoint Presentation

... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: August 27, 2016 - Views: 1

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PowerPoint Presentation

Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: August 27, 2016 - Views: 1

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 28, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: August 18, 2016 - Views: 2

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PowerPoint Presentation

... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs ...

http://genemol.org/molecular-bio/technics.ppt

Date added: August 21, 2016 - Views: 1

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No Slide Title

... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: August 27, 2016 - Views: 1

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: August 19, 2016 - Views: 1

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. Brief Chapter Outline I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: August 18, 2016 - Views: 1

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www.cell.com

... (DUP25) region showing STSs and genes surrounding the BACs and cosmids used as FISH probes (Gratacòs et al. 2001). The centromeric (CEN) and telomeric ...

http://www.cell.com/ajhg/ppt/S0002-9297(07)60569-2.ppt

Date added: August 29, 2016 - Views: 1

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Foundations of Biology - Geoscience Research...

Foundations of Biology ... Drosophila Genome Chromosome Morphology Chromosome Morphology Naming Chromosome Parts Drosophila X Chromosome 2b Cosmids An ...

http://grisda.org/resources/Power_Points/Molecular_Genetics/Fly_Genome.ppt

Date added: August 18, 2016 - Views: 1

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DUAL TRAFFICKING PATHWAYS OF CONNEXINS TO GAP...

... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...

http://www.swansea.ac.uk/media/media,35187,en.ppt

Date added: August 21, 2016 - Views: 1

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PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: August 20, 2016 - Views: 1

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Presentazione standard di PowerPoint - Unife

Cloning in Cosmids. Production of overlapping restriction fragments by partial digestion of human genomic DNA with Sau3A. CHROMOSOME WALKING. CHROMOSOME WALKING.

http://m.docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/5-genoteche-cdnateche.pptx

Date added: August 21, 2016 - Views: 1

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Die Achterbahn - Swansea University

... in E. coli Recombinant cosmid-DNA can easily be confirmed by PCR, restriction analysis and/or sequencing Mutagenised cosmids can be mobilised by conjugation, ...

http://www.swansea.ac.uk/media/media,35188,en.ppt

Date added: August 30, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: August 27, 2016 - Views: 1

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No Slide Title

Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: August 20, 2016 - Views: 1

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: August 30, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%202%202009.ppt

Date added: August 18, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids * * ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture309.ppt

Date added: August 18, 2016 - Views: 1

ppt
PowerPoint Presentation

AD000092 /FEATURE=cds#1 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, ...

http://combio.cs.brandeis.edu/hong/CS230a/CourseInfo/DetectDEG.ppt

Date added: August 20, 2016 - Views: 1

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HOW TO PREPARE VECTORS TO CARRY YOUR FAVORITE...

Biology 417 Week 1, Lecture #2 With input from: Yung Huang, Luis Sanchez, Lee Lin, Leticia Argueta, Kay Nguyen PGM 2000 Revised 2012 - SBS

http://www.calstatela.edu/faculty/ssharp/slides/%21PGM2012%21/12BiotechRE2012.ppt

Date added: August 30, 2016 - Views: 1

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Udspaltning af den recessive q = 0,01 og p = 0,99...

Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...

http://www.ihh.kvl.dk/htm/kc/popgen/genetics/10/10/kap10e.PPT

Date added: August 18, 2016 - Views: 1

ppt
PowerPoint Presentation

Cosmids(50kb) : وکتورهای ...

http://www.foodclinic.ir/wp-content/uploads/2016/04/tenth-session-biotechnology.pptx

Date added: August 30, 2016 - Views: 1

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Vectors - College of Computer, Mathematical, and...

Recombinant DNA Technology in Today’s Medicine Shelley M. Martineau Jessica A. Matthews Catherine C. Miller Carol D. Riley Institute of TIP Productions, Inc.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: August 22, 2016 - Views: 1

ppt
PowerPoint Presentation

Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show

http://mis.agri.cmu.ac.th/download/course/lec_359401_03%BE%D1%B9%B8%D8%C7%D4%C8%C7%A1%C3%C3%C1%C8%D2%CA%B5%C3%EC.ppt

Date added: August 29, 2016 - Views: 1

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Microarray-based Disease Prognosis using Gene...

Microarray-based Disease Prognosis using Gene Annotation Signatures Michael Kovshilovsky Swapna Annavarapu SoCalBSI 2005

http://instructional1.calstatela.edu/jmomand2/2005/presentations/ppt/annavarapu_kovshilovsky.ppt

Date added: August 18, 2016 - Views: 1

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Genética Molecular em Medicina Transfusional

Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...

http://homepage.ufp.pt/jcabeda/slides/gmed/gmed_sequenciacao.ppt

Date added: August 30, 2016 - Views: 1

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: August 30, 2016 - Views: 1

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justc/courses/RecombDNA/Week6. ppt - Portland...

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: August 19, 2016 - Views: 1

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Information Encoding in Biological Molecules: DNA...

2.2 - Microarray Data Modeling Lab Sébastien Lemieux Elitra Canada Ltd. Objectives to learn the basic principles about microarray experiments. to understand the main ...

http://bioinformatics.ca/dtt2004-lectures-day2-22sercbwppt

Date added: August 25, 2016 - Views: 1

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Chapter 12

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: August 30, 2016 - Views: 1

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DNA cloning intro.ppt - Biology

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: August 23, 2016 - Views: 1

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Cosmids can accept even larger inserts—up to 50 kb—making them a favorite choice for genomic libraries.

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: August 30, 2016 - Views: 1

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Chapter 4

... from isolated mRNAs 7.2 Preparation of a bacteriophage cDNA library 7.2 Larger DNA fragments can be cloned in cosmids and other vectors Screening ...

http://a32.lehman.cuny.edu/webwurtzel/course/Graduate%20lectures/lecture%205/lecture%205_Recombinant%20DNA%20Techniques.pps

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: August 22, 2016 - Views: 1

ppt
PowerPoint Presentation

... of 2 million plus reads derived from low copy BACs defined by Dr. Tabata et al. 3) identification of low copy cosmids (US) ...

https://solgenomics.net/static_content/solanaceae-project/sol-bioinformatics/pag2007_docs/pag2007_jg_bacext.ppt

Date added: August 30, 2016 - Views: 1

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www.cell.com

The cosmids from within the IGLL locus that are used for FISH are indicated on the top line over the region shaded in light red.

http://www.cell.com/ajhg/ppt/S0002-9297(07)62073-4.ppt

Date added: August 30, 2016 - Views: 1

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www.cell.com

Because the relative orientation of these cosmids is unknown, the gene order shown here is arbitrary along the chromosome but correct within the contig.

http://www.cell.com/trends/genetics/ppt/S0168-9525(04)00276-8.ppt

Date added: August 30, 2016 - Views: 1

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Setting up a transformation--how will the...

Setting up a transformation--how will the competent cells be treated? No plasmid (negative control, nothing should grow on this plate) Supercoiled plasmid of a known ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Title: PowerPoint Presentation Author: Mike Bartlett Last modified by: Justin Courcelle Created Date: 3/31/2003 6:09:42 AM Document presentation format

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: August 22, 2016 - Views: 1

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Forward Genetics - University of Colorado Boulder

Forward Genetics Survey ... mor-2 sup-23 evl-7 Genetic map map unit Cloned genes Non-cloned genes mDf4 nDf41 rme-2 unc-5 fem-1 skn-1 Physical map cosmids YACs ...

http://www.colorado.edu/MCDB/HanLab/part2.baisc_forward_genetics.ppt

Date added: August 23, 2016 - Views: 1

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Enzyme Mechanisms - Illinois Institute of...

Enzyme Mechanisms Author: ... used A typical plasmid Building useful plasmids Inserts Directional cloning Use of bacteriophage lambda Cosmids Cosmids ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: August 18, 2016 - Views: 1

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www.cell.com

... who has the 1.5-Mb deletion, were hybridized with probes from cosmids 84F7 and 62C4 (fluorescein and rhodamine, respectively; A and C) or cosmids 48C12 and 62C4 ...

http://www.cell.com/ajhg/ppt/S0002-9297(07)61712-1.ppt

Date added: August 30, 2016 - Views: 1

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PowerPoint Presentation - DNA Libraries

Title: PowerPoint Presentation - DNA Libraries Author: Jeffery Young Last modified by: Jeff Young Created Date: 10/23/2009 5:04:36 PM Document presentation format

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: August 23, 2016 - Views: 1

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Fragile X Syndrome - University of Wisconsin–Eau...

Fragile X Syndrome (Martin-Bell ... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids ...

http://people.uwec.edu/anderju/biol409/presentations/Fragile%20X%20Syndrome.ppt

Date added: August 30, 2016 - Views: 1

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www.cell.com

Human Origins of DNA Replication Selected from a Library of Nascent DNA Vesna Todorovic, Sara Giadrossi, Cristina Pelizon, Ramiro Mendoza-Maldonado, Hisao Masai ...

http://www.cell.com/molecular-cell/ppt/S1097-2765(05)01443-7.ppt

Date added: August 30, 2016 - Views: 1